Staphylococcus Aureus

WHAT IS IT?

Staph aureus is a gram positive coccal bacterium. The bacterial spheres aggregate together (like a literal staff).

Gram stain of S. aureus (source)
Gram stain of S. aureus (source)
CULTURE CHARACTERISTICS 

β-hemolytic bacteria: form clear area of hemolysis on blood agar

Alpha and beta hemolysis on blood agar (source)
Alpha and beta hemolysis on blood agar (source)

Catalase positive: bubbles observed when bacteria are added to hydrogen peroxide

Coagulase positive: this bacteria will cause plasma to coagulate/gel (in a tube) and will agglutinate/clump on a slide after citrated plasma is added.

MOLECULAR CHARACTERISTICS 

Protein A (virulence factor): binds Fc-IgG, and inhibits both complement activation and phagocytosis.

Toxic shock syndrome toxin: this enterotoxin type B is found in S. aureus and is a superantigen that binds to MHC II and T-cell receptor (activating them), ultimately resulting in polyclonal T-cell activation on a very large scale.

Exfoliative toxin: these extremely specific serine proteases recognize and cleave desmosomal cadherins only in the superficial layers of the skin, which is directly responsible for the clinical manifestation of staphylococcal scalded skin syndrome (SSSS), source

Preformed enterotoxin: this pre-formed toxin produced by S. aureus can cause food poisoning

TRANSMISSION
S. aureus is normally found colonizing the nose and can be transmitted by contact/displacement with/of the local pathogen.
CONDITIONS CAUSED

Dermatological: mastitis, impetigo, cellulitis, MRSA, scalded skin syndrome, general skin infections

Musculoskeletal: osteomyelitis

Gastrointestinal: food poisoning

Pulmonary: pneumonia

Cardiological: endocarditis (can occur from tunneled catheter)

Rheumatological: infective arthritis

Multisystem: abscess, wound infection, toxic shock syndrome (TSST-1), lymphadenitis

TREATMENT 

*Penicillins can be used

Cephalosporins used in general: Bactericidal β-lactam drugs that inhibit cell wall synthesis but are less susceptible to penicillinases.

Dicloxacillin, nafcillin, oxacillin: all used for non MRSA S. aureus treatment (MRSA has an altered penicillin-binding protein target site)

Cefazolin: prophylaxis for prevention of post surgical infections.

Daptomycin: Lipopeptide that disrupts cell membrane of gram-positive cocci. Used especially for MRSA.

Vancomycin: inhibits cell wall synthesis of gram positive bacteria. Used especially for MRSA.

Linezolid and ceftaroline also used for MRSA

OTHER HY FACTS

Commonly causes infection in chronic granulomatous disease

Most common cause of secondary pneumonia (bacterial pneumonia superimposed on a viral upper respiratory tract infection).

ARCHIVE OF STANDARDIZED EXAM QUESTIONS 

This archive compiles standardized exam questions that relate to this topic.

 

Page Updated: 01.28.2016